Title: Mode of de-esterification of alkaline and acidic pectin methyl esterases at different pH conditions
Authors: Duvetter, Thomas ×
Fraeye, Ilse
Sila, Daniel N
Verlent, Isabel
Smout, Chantal
Hendrickx, Marc
Van Loey, Ann #
Issue Date: Jan-2006
Publisher: Amer chemical soc
Series Title: Journal of Agricultural and Food Chemistry vol:54 issue:20 pages:7825-7831
Abstract: Highly esterified citrus pectin was de-esterified at pH 4.5 and 8.0 by a fungal pectin methyl esterase ( PME) that was shown to have an acidic isoelectric pH ( p I) and an acidic pH optimum and by a plant PME that was characterized by an alkaline p I and an alkaline pH optimum. Interchain and intrachain de-esterification patterns were studied by digestion of the pectin products with endo-polygalacturonase and subsequent analysis using size exclusion and anion-exchange chromatography. No effect of pH was observed on the de-esterification mode of either of the two enzymes. Acidic, fungal PME converted pectin according to a multiple-chain mechanism, with a limited degree of multiple attack at the intrachain level, both at pH 4.5 and at pH 8.0. A multiple-attack mechanism, with a high degree of multiple attack, was more appropriate to describe the action mode of alkaline, plant PME, both at pH 4.5 and at pH 8.0.
ISSN: 0021-8561
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Quantum Chemistry and Physical Chemistry Section
× corresponding author
# (joint) last author

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