Title: Protein-induced changes during the maturation process of human dendritic cells: A 2-D DIGE approach
Authors: Ferreira, Gabriela B
Overbergh, Lutgart ×
van Etten, Evelyne
Lage, Kasper
D'Hertog, Wannes
Hansen, Daniel Aaen
Maris, Michael
Moreau, Yves
Workman, Christopher T
Waelkens, Etienne
Mathieu, Chantal #
Issue Date: Sep-2008
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
Series Title: Proteomics - Clinical Applications vol:2 issue:9 pages:1349-1360
Article number: 10.1002/prca.200800110
Abstract: Dendritic cells (DCs) are unique antigen presenting cells, which upon maturation change from a specialized antigen-capturing cell towards a professional antigen presenting cells. In this study, a 2-D DIGE analysis of immature and mature DCs was performed, to identify proteins changing in expression upon maturation. The protein expression profile of immature and mature DCs, derived from CD14(+) peripheral blood monocytes was investigated using two pH ranges (pH 4-7 and 6-9) (n = 4). Ninety one differentially expressed spots (p<0.01) were detected, from which we identified 74 spots (81.32%) corresponding to 41 different proteins. The proteins identified play a role in diverse processes, such as antigen processing/presentation, vesicle transport and cytoskeleton remodeling. In addition, a protein interaction network contained 29 (out of 41) proteins, suggesting that, although they functionally originate from distinct classes, these proteins are acting as a protein-interactome. In conclusion, the proteins shown here to be altered in expression upon maturation are in line with the morphological and functional changes observed during the maturation process, providing a better understanding of the processes involved. This will open new avenues for investigating treatment regimens for immune-associated disorders.
ISSN: 1862-8346
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Clinical and Experimental Endocrinology
Molecular Virology and Gene Therapy
Laboratory of Phosphoproteomics (-)
ESAT - STADIUS, Stadius Centre for Dynamical Systems, Signal Processing and Data Analytics
Laboratory of Protein Phosphorylation and Proteomics
Medical Clerkships Centers
× corresponding author
# (joint) last author

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