Title: Ligand stoichiometry of the cold- and menthol-activated channel TRPM8
Authors: Janssens, Annelies
Voets, Thomas # ×
Issue Date: Oct-2011
Publisher: Cambridge University Press
Series Title: Journal of Physiology-London vol:589 issue:20 pages:4827-4835
Abstract: Temperature-sensitive TRP channels play a key role in somatosensation, not only as primary thermosensors but also as chemosensors for various compounds that evoke a thermal sensation. The fundamental mechanisms whereby TRP channels translate ligand binding into opening of the ion conducting pore are however poorly understood, and the actual number of ligands that bind to these channels is fully unknown. Here, we investigated the ligand stoichiometry of the cold sensor and menthol receptor TRPM8. Based on a detailed biophysical analysis of tandem constructs of wild-type and menthol-insensitive TRPM8 subunits, we now provide direct evidence that each channel has four independent and energetically equivalent menthol interaction sites. These results suggest a fundamentally different ligand stoichiometry in TRP channels, in comparison with other major families of ligand-gated ion channels.
ISSN: 0022-3751
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research (VIB-KU Leuven Center for Brain & Disease Research)
× corresponding author
# (joint) last author

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