Title: NMR of all-carbon-13 sugars: An application in development of an analytical method for a novel natural sugar, 1,5-anhydrofructose
Authors: Kametani, S ×
Mizuno, Hideaki
Shiga, Y
Akanuma, H #
Issue Date: Jan-1996
Publisher: Japanese Biochemical Society
Series Title: Journal of Biochemistry vol:119 issue:1 pages:180-185
Abstract: Of the all-carbon-13 compounds, glucose is one of the most easily accessible, and therefore we applied C-13-NMR technique to the metabolic study of glucose-related compounds, 1,5-anhydro-D-glucitol and 1,5-anhydro-D-fructose (AF). Applying an INADEQUATE method to the substitutes of these novel sugars fully labeled with carbon-13, we could trace out the entire carbon skeleton with high sensitivity and confirm the chemical structures of these sugars. The method also provided a much easier way to optimize the enzymatic oxidation for AF preparation: we selectively and continuously monitored the quantities, as well as their structures in aqueous solution, of the substrate and products in a noninvasive manner. Similarly relying upon information from the C-13-NMR, we developed a valuable derivatization method of AF for its GC-MS application, which was so sensitive that we were able to demonstrate the natural occurrence of AF in rat liver.
ISSN: 0021-924X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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