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Title: Regulated fast nucleocytoplasmic shuttling observed by reversible protein highlighting
Authors: Ando, R ×
Mizuno, Hideaki
Miyawaki, A #
Issue Date: Nov-2004
Publisher: American Association for the Advancement of Science
Series Title: Science vol:306 issue:5700 pages:1370-1373
Abstract: The observation of the regulation of fast protein dynamics in a cellular context requires the development of reliable technologies. Here, a signal regulation cascade reliant on the stimulus-dependent acceleration of the bidirectional flow of mitogen-activated protein kinase (extracellular signal-regulated kinase) across the nuclear envelope was visualized by reversible protein highlighting. Light-induced conversion between the bright and dark states of a monomeric fluorescent protein engineered from a novel coral protein was employed. Because of its photochromic properties, the protein could be highlighted, erased, and highlighted again in a nondestructive manner, allowing direct observation of regulated fast nucleocytoplasmic shuttling of key signaling molecules.
URI: 
ISSN: 0036-8075
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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