Title: Attenuation of photobleaching in two-photon excitation fluorescence from green fluorescent protein with shaped excitation pulses
Authors: Kawano, H ×
Nabekawa, Y
Suda, A
Oishi, Y
Mizuno, Hideaki
Miyawaki, A
Midorikawa, K #
Issue Date: Nov-2003
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:311 issue:3 pages:592-596
Abstract: The two-photon excitation fluorescence (TPEF) process of an enhanced green fluorescent protein (EGFP) for fluorescence signals was adaptively controlled by the phase-modulation of femtosecond pulses. After the iteration of pulse shaping, a twofold increase in the ratio of the fluorescence signal to the laser peak power was achieved. Compared with conventional pulses optimized for peak power, phase-optimized laser pulses reduced the bleaching rate of EGFP by a factor of 4 while maintaining the same intensity of the fluorescence signal. Our method will provide a powerful solution to various problems confronting researchers, such as the photobleaching of dyes in two-photon excitation microscopy. (C) 2003 Elsevier Inc. All rights reserved.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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