Title: FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule
Authors: Truong, K ×
Sawano, A
Mizuno, Hideaki
Hama, H
Tong, KI
Mal, TK
Miyawaki, A
Ikura, M #
Issue Date: Dec-2001
Publisher: Nature Pub. Co.
Series Title: Nature Structural Biology vol:8 issue:12 pages:1069-1073
Abstract: Intracellular Ca2+ acts as a second messenger that regulates numerous physiological cellular phenomena including development, differentiation and apoptosis. Cameleons, a class of fluorescent indicators for Ca2+ based on green fluorescent proteins (GFPs) and calmodulin (CaM), have proven to be a useful tool in measuring free Ca2+ concentrations in living cells. Traditional cameleons, however, have a small dynamic range of fluorescence resonance energy transfer (FRET), making subtle changes in Ca2+ concentrations difficult to detect and study in some cells and organelles. Using the NMR structure of CaM bound to the CaM binding peptide derived from CaM-dependent kinase kinase (CKKp), we have rationally designed a new cameleon that displays a twofold increase in the FRET dynamic range within the physiologically significant range of cytoplasmic Ca2+ concentration of 0.05-1 muM.
ISSN: 1072-8368
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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