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Title: Crystallographic evidence for water-assisted photo-induced peptide cleavage in the stony coral fluorescent protein kaede
Authors: Hayashi, Ikuko ×
Mizuno, Hideaki
Tong, Kit I
Furuta, Toshiaki
Tanaka, Fujie
Yoshimura, Masato
Miyawaki, Atsushi
Ikura, Mitsuhiko #
Issue Date: Sep-2007
Publisher: Academic Press
Series Title: Journal of Molecular Biology vol:372 issue:4 pages:918-926
Abstract: A coral fluorescent protein from Trachyphyllia geoffroyi, Kaede, possesses a tripeptide of His62-Tyr63-Gly64, which forms a chromophore with green fluorescence. This chromophore's fluorescence turns red following UV light irradiation. We have previously shown that such photoconversion is achieved by a formal beta-elimination reaction, which results in a cleavage of the peptide bond found between the amide nitrogen and the a-carbon at His62. However, the stereochemical arrangement of the chromophore and the precise structural basis for this reaction mechanism previously remained unknown. Here, we report the crystal structures of the green and red form of Kaede at 1.4 angstrom and 1.6 angstrom resolutions, respectively. Our structures depict the cleaved peptide bond in the red form. The chromophore conformations both in the green and red forms are similar, except a well-defined water molecule in the proximity of the His62 imidazole ring in the green form. We propose a molecular mechanism for green-to-red photoconversion, which is assisted by the water molecule. (c) 2007 Elsevier Ltd. All rights reserved.
URI: 
ISSN: 0022-2836
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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