Title: Hidden electronic excited state of enhanced green fluorescent protein
Authors: Hosoi, Haruko ×
Yarnaguchi, Shoichi
Mizuno, Hideaki
Miyawaki, Atsushi
Tahara, Tahei #
Issue Date: Mar-2008
Publisher: American Chemical Society
Series Title: Journal of Physical Chemistry B vol:112 issue:10 pages:2761-2763
Abstract: Precise two-photon absorption spectra of the green fluorescent protein (GFP) and the mutants sapphire-GFP (T203I) and enhanced GFP (S65T/F64L), as well as a model compound for the chromophore, 4'-hydroxybenzylidene-2,3-dimethylimidazolinone (HBDI) were measured by multiplex two-photon absorption spectroscopy. The observed TPA bands of the anionic forms of enhanced GFP and HBDI were significantly shifted to the higher energy compared with the lowest-energy bands in one-photon absorption spectra. This result indicated the existence of a hidden electronic excited state in the vicinity of the lowest excited singlet (S I) state of the anionic form of the GFP chromophore, which is the origin of the blue shift of the two-photon absorption spectra as well as two-photon fluorescence excitation spectra.
ISSN: 1520-6106
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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