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Title: Identification of a novel autoantigen in inflammatory bowel disease by protein microarray
Authors: Vermeulen, Nathalie
Op de Béeck, Katrijn
Vermeire, Severine
Van Steen, Kristel
Michiels, Georges
Ballet, Vera
Rutgeerts, Paul
Bossuyt, Xavier # ×
Issue Date: Jun-2011
Publisher: John Wiley & Sons
Series Title: Inflammatory Bowel Diseases vol:17 issue:6 pages:1291-1300
Article number: 10.1002/ibd.21508
Abstract: BACKGROUND:: Patients with inflammatory bowel disease (IBD) display immunoreactivity to self-antigens and microbial antigens. We used a protein microarray approach to identify novel autoantigens in IBD. METHODS:: ProtoArray Human Protein Microarray v4.0 containing 8268 human proteins from Invitrogen (La Jolla, CA) was used. RESULTS:: Twenty-five IBD patients and five healthy controls were screened for candidate autoantigens. For 256 antigens, IBD patients had a higher seroreactivity than controls. Twenty antigens were selected for further evaluation in a larger cohort (60 ulcerative colitis [UC] patients, 60 Crohn's disease [CD] patients, 60 healthy controls, and 60 gastrointestinal-diseased controls) by means of a customized protein microarray. Out of these 20 antigens, one antigen, family with sequence similarity 84 member A (FAM84A), was identified as a target antigen in IBD. Antibodies to FAM84A were significantly more prevalent in IBD patients (19%) than in gastrointestinal-diseased controls (1.7%) (P = 0.0008) and healthy controls (5%) (P = 0.01). Anti-FAM84A antibodies were found in 26.6% of UC patients and in 11.7% of CD patients. FAM84A was confirmed as target antigen in IBD by means of Western blotting in a large independent cohort (100 UC patients, 106 CD patients, 102 healthy controls, and 100 gastrointestinal-diseased controls). Antibodies to FAM84A were significantly more prevalent in IBD patients (20%) than in gastrointestinal-diseased controls (5%) (P = 0.0004) and healthy controls (0%) (P < 0.0001). Anti-FAM84A antibodies were found in 18% of UC patients and in 22% of CD patients. CONCLUSIONS:: We identified FAM84A as a novel autoantigen in IBD. (Inflamm Bowel Dis 2010;).
URI: 
ISSN: 1078-0998
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical Bacteriology and Mycology
Department of Human Genetics - miscellaneous
Translational Research in GastroIntestinal Disorders
Experimental Laboratory Immunology
× corresponding author
# (joint) last author

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