Title: Single-cell copy number variation detection
Authors: Cheng, Jiqiu
Vanneste, Evelyne
Konings, Peter
Voet, Thierry
Vermeesch, Joris
Moreau, Yves # ×
Issue Date: Aug-2011
Publisher: BioMed Central Ltd.
Series Title: Genome Biology vol:12 issue:8
Article number: R80
Abstract: ABSTRACT: Detection of chromosomal aberrations from a single cell by array comparative genomic hybridization (single-cell array CGH), instead of from a population of cells, is an emerging technique. However, such detection is challenging because of the genome artifacts and the DNA amplification process inherent to the single cell approach. Current normalization algorithms result in inaccurate aberration detection for single-cell data. We propose a normalization method based on channel, genome composition and recurrent genome artifact corrections. We demonstrate that the proposed channel clone normalization significantly improves the copy number variation detection both in simulated and real single-cell array CGH data.
ISSN: 1465-6906
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
ESAT - STADIUS, Stadius Centre for Dynamical Systems, Signal Processing and Data Analytics
Laboratory for Cytogenetics and Genome Research
× corresponding author
# (joint) last author

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