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Title: Covalent enzyme immobilization on paramagnetic polyacrolein beads
Authors: Varlan, AR ×
Sansen, Willy
Van Loey, Ann
Hendrickx, Marc #
Issue Date: Jan-1996
Publisher: Elsevier advanced technology
Series Title: Biosensors & bioelectronics vol:11 issue:4 pages:443-448
Abstract: An optimized procedure of covalent glucose oxidase, urease, Bacillus subtilis alpha-amylase and Bacillus licheniformis alpha-amylase immobilization on paramagnetic, non-porous, polyacrolein beads is presented. The resulting insolubilized enzymes can be employed for extended periods of time without loss of activity. The conditions were optimized for maximizing the activity of the linked enzyme. Coated beads bearing up to 15 mu g(active) (enzyme)/mg(beads) were obtained on reproducible basis. The paramagnetic feature of the particles facilitates the enzyme handling. In the magnetic field, the enzyme separation is fast and complete. Thus, the paramagnetic beads represent an excellent carrier for immobilized enzymes.
ISSN: 0956-5663
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre for Food and Microbial Technology
Electrical Engineering - miscellaneous
× corresponding author
# (joint) last author

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