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Title: Detection of antinuclear antibodies by indirect immunofluorescence and by solid phase assay
Authors: Op De Beeck, Katrijn
Vermeersch, Pieter
Verschueren, Patrick
Westhovens, René
Mariën, Godelieve
Blockmans, Daniel Engelbert
Bossuyt, Xavier # ×
Issue Date: Oct-2011
Publisher: Elsevier
Series Title: Autoimmunity Reviews vol:10 issue:12 pages:801-808
Abstract: Testing for antinuclear antibodies is useful for the diagnosis of systemic rheumatic diseases. Solid phase assays are increasingly replacing indirect immunofluorescence for detection of antinuclear antibodies. In the most recent generation of solid phase assays, manufacturers attempt to improve the performance of the assays by adding extra antigens. Solid phase assay (EliA CTD Screen, Phadia, in which antibodies to 17 antigens are detected) was compared to indirect immunofluorescence for the detection of antinuclear antibodies in diagnostic samples of 236 patients with autoimmune connective tissue diseases, in 149 healthy blood donors, 139 patients with chronic fatigue syndrome, and 134 diseased controls. The sensitivity of EliA CTD Screen for systemic lupus erythematosus, systemic sclerosis, primary Sjögren's syndrome, mixed connective tissue disease, and inflammatory myopathy was 74%, 72%, 89%, 100%, and 39%, respectively. The reactivity in blood donors, in patients with chronic fatigue syndrome, and in diseased controls was <4%. At an immunofluorescence cutoff that corresponded to the specificity found with solid phase assays, the sensitivity of indirect immunofluorescence was lower than the sensitivity of solid phase assays. Likelihood ratios increased with increasing antibody concentrations. Generally, a positive test result by EliA CTD Screen had a higher likelihood ratio for systemic rheumatic disease than a positive test result by indirect immunofluorescence. A negative test result by indirect immunofluorescence, however, had a lower likelihood ratio than a negative test result by EliA CTD Screen, indicating that the negative predictive value was higher for indirect immunofluorescence than for EliA CTD screen.
URI: 
ISSN: 1568-9972
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Clinical Infectious and Inflammatory Disorders
Cardiology
Experimental Laboratory Immunology
Neuro-musculo-skeletal Research (-)
× corresponding author
# (joint) last author

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