Title: Apparent heterogeneity in the pIII-peptide fusion protein in single-phage clones isolated from peptide libraries
Authors: Noppe, Wim
Galaev, Igor Yu
Mattiasson, Bo
Deckmyn, Hans # ×
Issue Date: Jun-2011
Publisher: Oxford University Press
Series Title: Protein Engineering, Design & Selection vol:24 pages:721-726
Abstract: Ligand homogeneity is an important issue in affinity chromatography. Using phages expressing peptides on the pIII protein, a heterogeneity in the binding of monoclonal phages was observed during affinity chromatography on supermacroporous cryogels. Fractions with different apparent binding affinities could be separated by stepwise elution. When these different fractions were re-applied, the respective differences in affinity were retained. However, when phage fractions with different apparent affinities were first amplified, an offspring was generated with again variable affinities. As the sequence of the peptide insert was the same, the heterogeneity must be ascribed to differences in avidity and although no direct evidence could be generated, we hypothesize that this is possibly due to phages displaying different numbers of the same peptide as a consequence of either proteolytic or packaging events during the amplification step in Escherichia coli.
ISSN: 1741-0126
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Chemistry, Campus Kulak Kortrijk
× corresponding author
# (joint) last author

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