Title: Stem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland
Authors: Anderson, Lisa H
Boulanger, Corinne A
Smith, Gilbert H
Carmeliet, Peter
Watson, Christine J # ×
Issue Date: Mar-2011
Publisher: John Wiley & Sons
Series Title: Developmental Dynamics vol:240 issue:3 pages:674-681
Article number: 10.1002/dvdy.22539
Abstract: Prominin-1 (Prom1) is recognized as a stem cell marker in several tissues, including blood, neuroepithelium, and gut, and in human and mouse embryos and many cancers. Although Prom1 is routinely used as a marker for isolating stem cells, its biological function remains unclear. Here we use a knockout model to investigate the role of Prom1 in the mammary gland. We demonstrate that complete loss of Prom1 does not affect the regenerative capacity of the mammary epithelium. Surprisingly, we also show that in the absence of Prom1, mammary glands have reduced ductal branching, and an increased ratio of luminal to basal cells. The effects of Prom1 loss in the mammary gland are associated with decreased expression of prolactin receptor and matrix metalloproteinase-3. These experiments reveal a novel, functional role for Prom1 that is not related to stem cell activity, and demonstrate the importance of tissue-specific characterization of putative stem cell markers.
ISSN: 1058-8388
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Vesalius Research Centre (-)
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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