Title: Designing optimal calcium phosphate scaffold-cell combinations using an integrative model based approach
Authors: Carlier, AurĂ©lie
Chai, Yoke Chin
Moesen, Maarten
Theys, Tina
Schrooten, Jan
Van Oosterwyck, Hans
Geris, Liesbet # ×
Issue Date: 2011
Publisher: Elsevier
Series Title: Acta Biomaterialia vol:7 issue:10 pages:3573-3585
Abstract: Bone formation is a very complex physiological process, involving the participation of many different cell types and regulated by countless biochemical, physical and mechanical factors, including naturally occurring or synthetic biomaterials. For the latter calcium phosphate (CaP) based scaffolds have proven to stimulate bone formation, but at present still result in a wide range of in vivo outcomes, which is partly related to the sub-optimal use and combination with osteogenic cells. To optimize CaP scaffold selection and make their use in combination with cells more clinically relevant this study uses an integrative approach in which mathematical modeling is combined with experimental research. This paper describes the development and implementation of an experimentally informed bioregulatory model of the effect of calcium ions released from CaP-based biomaterials on the activity of osteogenic cells and mesenchymal stem cell driven ectopic bone formation.
The amount of bone formation predicted by the mathematical model corresponds to the amount measured experimentally under similar conditions. Moreover, the model is also able to qualitatively predict the experimentally observed impaired bone formation under conditions such as insufficient cell seeding and scaffold decalcification. A strategy was designed in silico to overcome the negative influence of a low initial cell density on the bone formation process. Finally, the model was applied to design optimal combinations of calcium-based biomaterials and cell culture conditions with the aim of maximizing the amount of bone formation. This work illustrates the potential of mathematical models as research tools to design more efficient and cell-customized CaP scaffolds for bone tissue engineering applications.
ISSN: 1742-7061
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biomechanics Section
Rheumatology Section (-)
Department of Materials Engineering - miscellaneous
× corresponding author
# (joint) last author

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