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Title: Biofunctionalization of electrowetting-on-dielectric digital microfluidic chips for miniaturized cell-based applications
Authors: Witters, Daan
Vergauwe, Nicolas
Vermeir, Steven
Ceyssens, Frederik
Liekens, Sandra
Puers, Bob
Lammertyn, Jeroen # ×
Issue Date: Aug-2011
Publisher: Royal Society of Chemistry
Series Title: Lab on a Chip vol:11 issue:16 pages:2790-2794
Abstract: In this paper we report on the controlled biofunctionalization of the hydrophobic layer of
electrowetting-on-dielectric (EWOD) based microfluidic chips with the aim to execute (adherent) cellbased
assays. The biofunctionalization technique involves a dry lift-off method with an easy to remove
Parylene-C mask and allows the creation of spatially controlled micropatches of biomolecules in the
Teflon-AF layer of the chip. Compared to conventional methods, this method (i) is fully
biocompatible; and (ii) leaves the hydrophobicity of the chip surface unaffected by the fabrication
process, which is a crucial feature for digital microfluidic chips. In addition, full control of the geometry
and the dimensions of the micropatches is achieved, allowing cells to be arrayed as cell clusters or as
single cells on the digital microfluidic chip surface. The dry Parylene-C lift-off technique proves to have
great potential for precise biofunctionalization of digital microfluidic chips, and can enhance their use
for heterogeneous bio-assays that are of interest in various biomedical applications.
ISSN: 1473-0197
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Mechatronics, Biostatistics and Sensors (MeBioS)
ESAT - MICAS, Microelectronics and Sensors
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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