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Title: The correct use of "average" fluorescence parameters
Authors: Sillen, A ×
Engelborghs, Yves #
Issue Date: Jan-1998
Publisher: Amer soc photobiology
Series Title: Photochemistry and photobiology vol:67 issue:5 pages:475-486
Abstract: When more than one fluorophore is present or when one fluorophore displays a multiple exponential decay "average" fluorescence parameters are derived, which can be combined with "average" lifetimes for further interpretation. However, two kinds of average lifetimes are used in this context: the intensity and the amplitude average lifetime. In this paper the different average parameters are carefully analyzed and their "best" combinations are derived. These average parameters are analyzed in the context of external and internal dynamic and static quenching, Foster energy transfer and the calculation of the radiative rate constant. The use of the amplitude average lifetime for the analysis of multiple fluorophore-containing systems and the detection of interactions is discussed.
URI: 
ISSN: 0031-8655
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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