Synthesis of L-xylo-hexos-2-ulose (L-sorbosone) and its characterisation by chromatographic and spectroscopic techniques
Van der Eycken, Erik × Carlens, G Messens, J Zhao, YN Bauw, G Van der Eycken, J Sandra, P Van Montagu, M #
Elsevier science bv
Journal of Chromatography A vol:811 issue:1-2 pages:261-268
A modified pathway to synthesize on large scale L-xylo-hexos-2-ulose (L-sorbosone), a potential intermediate in the biosynthesis of L-ascorbic acid in some plants, is described. The determination of the purity by thin-layer chromatography, high-performance liquid chromatography, capillary gas chromatography (cGC) and cGC-mass spectrometry (MS) is discussed. The product is for the first time unambiguously characterized by C-13 NMR and electrospray MS. (C) 1998 Elsevier Science B.V. All rights reserved.