Title: In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly-L-lysine-treated titanium-6-aluminium-4-vanadium
Authors: Galli, Daniela ×
Benedetti, Laura
Bongio, Matilde
Maliardi, Valentina
Silvani, Giulia
Ceccarelli, Gabriele
Ronzoni, Flavio
Conte, Silvio
Benazzo, Francesco
Graziano, Antonio
Papaccio, Gianpaolo
Sampaolesi, Maurilio
De Angelis, Maria Gabriella Cusella #
Issue Date: May-2011
Publisher: John Wiley & Sons
Series Title: Journal of Biomedical Materials Research A vol:97A issue:2 pages:118-126
Abstract: Three-dimensional (3D) titanium-6-aluminium-4-vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high-mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture-treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly-L-lysine (poly-L-lys). Poly-L-lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly-L-lys alone induces FAK activation through beta 1-INTEGRIN, because the presence of beta 1-INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly-L-lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 97A: 118-126, 2011.
ISSN: 1549-3296
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdepartemental Stem Cell Institute (-)
× corresponding author
# (joint) last author

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