Title: Evaluation of the specificity and sensitivity of ferritin as an MRI reporter gene in the mouse brain using lentiviral and adeno-associated viral vectors
Authors: Vande Velde, Greetje
Rangarajan, Janaki Raman
Toelen, Jaan
Dresselaers, Tom
Ibrahimi, Abdelilah
Krylychkina, Olga
Vreys, Ruth
Van der Linden, Annemie
Maes, Frederik
Debyser, Zeger
Himmelreich, Uwe
Baekelandt, Veerle # ×
Issue Date: Jun-2011
Publisher: Macmillan Press Ltd.
Series Title: Gene Therapy vol:18 issue:6 pages:594-605
Article number: 10.1038/gt.2011.2
Abstract: The development of in vivo imaging protocols to reliably track transplanted cells or to report on gene expression is critical for treatment monitoring in (pre)clinical cell and gene therapy protocols. Therefore, we evaluated the potential of lentiviral vectors (LVs) and adeno-associated viral vectors (AAVs) to express the magnetic resonance imaging (MRI) reporter gene ferritin in the rodent brain. First, we compared the induction of background MRI contrast for both vector systems in immune-deficient and immune-competent mice. LV injection resulted in hypointense (that is, dark) changes of T(2)/T(2)(*) (spin-spin relaxation time)-weighted MRI contrast at the injection site, which can be partially explained by an inflammatory response against the vector injection. In contrast to LVs, AAV injection resulted in reduced background contrast. Moreover, AAV-mediated ferritin overexpression resulted in significantly enhanced contrast to background on T(2)(*)-weighted MRI. Although sensitivity associated with the ferritin reporter remains modest, AAVs seem to be the most promising vector system for in vivo MRI reporter gene imaging.
ISSN: 0969-7128
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Research Group for Neurobiology and Gene Therapy
Molecular Virology and Gene Therapy
ESAT - PSI, Processing Speech and Images
Biomedical MRI
× corresponding author
# (joint) last author

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