Title: Nanoleakage Distribution at Adhesive-Dentin Interfaces in 3D
Authors: Coutinho, E ×
Cardoso, M V
Fernandes, C P
Neves, A A
Gouvea, C V D
Van Landuyt, Kirsten
De Munck, Jan
Van Meerbeek, Bart #
Issue Date: Aug-2011
Publisher: Journal of Dental Research
Series Title: Journal of Dental Research vol:90 issue:8 pages:1019-1025
Abstract: In spite of its role in the degradation of tooth-biomaterial interfaces, reports on nanoleakage are largely inconsistent. The aim of this work was to assess nanoleakage patterns qualitatively and quantitatively in 3D, to determine the influence of direction, position, and inclination of the field-of-view. Therefore, we applied a gold-standard 3-step etch-and-rinse adhesive to bur-cut dentin surfaces, after which interface samples were sectioned, infiltrated with an ammoniacal silver-nitrate solution, and embedded by common TEM procedures. High-resolution 3D models of interfaces were then generated by FIB and electron tomography, following strict conditions determined by Monte Carlo simulations. Inverted images in FIB tomography disclosed morphological characteristics analogous to those revealed by TEM. Quantitative analysis revealed large variations in silver-nitrate uptake between 2D image projections in different directions. Furthermore, silver-nitrate fractions in individual 2D image projections were seldom related to the total 3D volumetric fraction. Electron tomography showed that inclination also affected the morphology of silver-nitrate patterns. In conclusion, conventional nanoleakage evaluation is heavily influenced by direction, position, and inclination of the field-of-view, and thus may contain artifacts.
ISSN: 0022-0345
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biomaterials - BIOMAT
× corresponding author
# (joint) last author

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