Title: From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family
Authors: Wiedenmann, Jörg
Gayda, Susan
Adam, Virgile
Oswald, Franz
Nienhaus, Karin
Bourgeois, Dominique
Nienhaus, G Ulrich # ×
Issue Date: Jun-2011
Publisher: Wiley-VCH Verlag GMBH
Series Title: Journal of Biophotonics vol:4 issue:6 pages:377-90
Article number: 10.1002/jbio.201000122
Abstract: Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ∼400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).
ISSN: 1864-063X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science