Title: Morphological, physiological and behavioural evaluation of a 'Mice in Space' housing system
Authors: Blottner, Dieter ×
Serradj, Najet
Salanova, Michele
Touma, Chadi
Palme, Rupert
Silva, Mitchell
Aerts, Jean-Marie
Berckmans, Daniel
Vico, Laurence
Liu, Yimin
Giuliani, Alessandra
Rustichelli, Franco
Cancedda, Ranieri
Jamon, Marc #
Issue Date: May-2009
Publisher: Springer-Verlag
Series Title: Journal of Comparative Physiology B, Biochemical, Systemic and Environmental Physiology vol:179 issue:4 pages:519-533
Abstract: Environmental conditions likely affect physiology and behaviour of mice used for life sciences research on Earth or in Space. Here, we analysed the effects of cage confinement on the weightbearing musculoskeletal system, behaviour and stress of wild-type mice (C57BL/6JRj, 30 g b.wt., total n = 24) housed for 25 days in a prototypical ground-based and fully automated life support habitat device called "Mice in Space" (MIS). Compared with control housing (individually ventilated cages) the MIS mice revealed no significant changes in soleus muscle size and myofiber distribution (type I vs. II) and quality of bone (3-D microarchitecture and mineralisation of calvaria, spine and femur) determined by confocal and micro-computed tomography. Corticosterone metabolism measured non-invasively (faeces) monitored elevated adrenocortical activity at only start of the MIS cage confinement (day 1). Behavioural tests (i.e., grip strength, rotarod, L/D box, elevated plus-maze, open field, aggressiveness) performed subsequently revealed only minor changes in motor performance (MIS vs. controls). The MIS habitat will not, on its own, produce major effects that could confound interpretation of data induced by microgravity exposure during spaceflight. Our results may be even more helpful in developing multidisciplinary protocols with adequate scenarios addressing molecular to systems levels using mice of various genetic phenotypes in many laboratories.
ISSN: 0174-1578
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division M3-BIORES: Measure, Model & Manage Bioresponses (-)
× corresponding author
# (joint) last author

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