Bulletin de la Société Belge d'Ophtalmologie vol:317 pages:55
Ophthalmologia Belgica Congress location:Brussels, Belgium date:23-25 November 2011
PURPOSE The aim of this project was to study the specific role of the VEGF
isoforms in pathological angiogenesis, and to investigate the effect of blocking
a single isoform on the formation of choroidal neovascularization (CNV).
METHODS To study the in vivo role of the VEGF isoforms in pathological
angiogenesis, VEGF isoform specific mice (VEGF 120/+, VEGF 164/164 and
VEGF188/188 mice) were bred. After backcrossing these mice strains into
a C75Bl/6 background CNV was induced by placing 3 green laser spots
(100μm spot size, 0.05 sec spot duration and 400mW power) around the
optic nerve. Quantification of the area of newly formed blood vessels was
determined by retrobulbar dextran linked FITC-perfusion.
RESULTS Preliminary results showed that the area of neovascularization
in the VEGF 120/+ and VEGF 164/164 mice was comparable to the wild
type mice, whereas an inhibition in neovascularization was present in
the VEGF188/188 isoform specific mice. For the moment, mice colonies
are being enlarged to repeat experiments and subsequently, these mice
are intercrossed to obtain double transgenic mice. Furthermore, retinal
angiogenesis will also be checked using the ROP model.
CONCLUSION VEGF164 and VEGF120 seems to play an important role
in the process of pathological neovascularization, whereas VEGF188 not.
These new insights in the role of different VEGF isoforms may improve
the efficacy of the currently existing anti-VEGF therapy (that differ in their
specificity for the different VEGF isoforms). This can open new perspectives
for patients with vision threatening eye diseases and may have important
therapeutic implications for them.