Title: Unique Spectrum of Activity of Prosimian TRIM5 alpha against Exogenous and Endogenous Retroviruses
Authors: Rahm, Nadia ×
Yap, Melvyn
Snoeck, Joke
Zoete, Vincent
Munoz, Miguel
Radespiel, Ute
Zimmermann, Elke
Michielin, Olivier
Stoye, Jonathan P
Ciuffi, Angela
Telenti, Amalio #
Issue Date: May-2011
Publisher: American Society for Microbiology (ASM)
Series Title: Journal of Virology vol:85 issue:9 pages:4173-4183
Abstract: Lentiviruses, the genus of retrovirus that includes HIV-1, rarely endogenize. Some lemurs uniquely possess an endogenous lentivirus called PSIV ("prosimian immunodeficiency virus"). Thus, lemurs provide the opportunity to study the activity of host defense factors, such as TRIM5 alpha, in the setting of germ line invasion. We characterized the activities of TRIM5 alpha proteins from two distant lemurs against exogenous retroviruses and a chimeric PSIV. TRIM5 alpha from gray mouse lemur, which carries PSIV in its genome, exhibited the narrowest restriction activity. One allelic variant of gray mouse lemur TRIM5 alpha restricted only N-tropic murine leukemia virus (N-MLV), while a second variant restricted N-MLV and, uniquely, B-tropic MLV (B-MLV); both variants poorly blocked PSIV. In contrast, TRIM5 alpha from ring-tailed lemur, which does not contain PSIV in its genome, revealed one of the broadest antiviral activities reported to date against lentiviruses, including PSIV. Investigation into the antiviral specificity of ring-tailed lemur TRIM5 alpha demonstrated a major contribution of a 32-amino-acid expansion in variable region 2 (v2) of the B30.2/SPRY domain to the breadth of restriction. Data on lemur TRIM5 alpha and the prediction of ancestral simian sequences hint at an evolutionary scenario where antiretroviral specificity is prominently defined by the lineage-specific expansion of the variable loops of B30.2/SPRY.
ISSN: 0022-538X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Clinical and Epidemiological Virology (Rega Institute)
× corresponding author
# (joint) last author

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