Title: Antiidiotypic and anti-anti-idiotypic antibodies as highly specific tools in epitope and active-site studies on human interferon-gamma
Authors: Depla, E ×
Louwagie, J
De Ley, Marc #
Issue Date: Jan-1991
Publisher: Pergamon-elsevier science ltd
Series Title: Molecular immunology vol:28 issue:7 pages:743-751
Abstract: Immunization of rabbits with F(ab')2 fragments of different monoclonal antibodies directed against human interferon-gamma yielded antisera with anti-idiotypic characteristics. Isolation of the anti-idiotypic fraction, resulting in a highly specific antiserum, allowed us to prove that out of six competing monoclonal antibodies directed against human interferon-gamma, only two really recognize the same epitope. The other monoclonal antibodies compete on the basis of steric hindrance, which is not surprising, because of the large difference in M, between interferon-gamma and an immunoglobulin.
ISSN: 0161-5890
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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