Title: Protein folding, unfolding and aggregation. Pressure induced intermediate states on the refolding pathway of horseradish peroxidase
Authors: Smeller, L ×
Fidy, J
Heremans, Karel #
Issue Date: Jan-2004
Publisher: Iop publishing ltd
Series Title: Journal of physics-condensed matter vol:16 issue:14 pages:S1053-S1058
Abstract: We studied the refolding and aggregation of pressure unfolded proteins. Horseradish peroxidase was found to be very stable and no partially folded intermediates were populated during the refolding. However, the removal of the haem group or the Ca2+ ions or reduction of the disulfide bridge destabilized the protein, resulting in a significant amount of aggregation prone intermediate conformation. Substitution of the haem for fluorescent porphyrin however did not influence the refolding of the protein.
ISSN: 0953-8984
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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