Title: Nucleobase recognition in ssDNA at the central constriction of the alpha-hemolysin pore
Authors: Stoddart, David
Heron, Andrew J
Klingelhoefer, Jochen
Mikhailova, Ellina
Maglia, Giovanni
Bayley, Hagan # ×
Issue Date: Sep-2010
Publisher: American Chemical Society
Series Title: Nano Letters vol:10 issue:9 pages:3633-3637
Abstract: Nanopores are under investigation for single-molecule DNA sequencing. The alpha-hemolysin (alphaHL) protein nanopore contains three recognition points capable of nucleobase discrimination in individual immobilized ssDNA molecules. We have modified the recognition point R(1) by extensive mutagenesis of residue 113. Amino acids that provide an energy barrier to ion flow (e.g., bulky or hydrophobic residues) strengthen base identification, while amino acids that lower the barrier weaken it. Amino acids with related side chains produce similar patterns of nucleobase recognition providing a rationale for the redesign of recognition points.
ISSN: 1530-6984
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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