Title: Controlled Translocation of Individual DNA Molecules through Protein Nanopores with Engineered Molecular Brakes
Authors: Rincon-Restrepo, Marcela
Milthallova, Ellina
Bayley, Hagan
Maglia, Giovanni # ×
Issue Date: Feb-2011
Publisher: American Chemical Society
Series Title: Nano Letters vol:11 issue:2 pages:746-750
Abstract: Protein nanopores may provide a cheap and fast technology to sequence individual DNA molecules. However, the electrophoretic translocation of ssDNA molecules through protein nanopores has been too rapid for base identification. Here, we show that the translocation of DNA molecules through the alpha-hemolysin protein nanopore can be slowed controllably by introducing positive charges into the lumen of the pore by site directed mutagenesis. Although the residual ionic current during DNA translocation is insufficient for direct base identification, we propose that the engineered pores might be used to slow down DNA in hybrid systems, for example, in combination with solid-state nanopores.
ISSN: 1530-6984
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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