Title: Redox Proteomics of Protein-bound Methionine Oxidation
Authors: Ghesquière, Bart ×
Jonckheere, Veronique
Colaert, Niklaas
Van Durme, Joost
Timmerman, Evy
Goethals, Marc
Schymkowitz, Joost
Rousseau, Frederic
Vandekerckhove, Joël
Gevaert, Kris #
Issue Date: May-2011
Publisher: American Society for Biochemistry and Molecular Biology
Series Title: Molecular & Cellular Proteomics vol:10 issue:5 pages:M110.006866
Abstract: We here present a new method to measure the degree of protein-bound methionine sulfoxide formation at a proteome-wide scale. In human Jurkat cells that were stressed with hydrogen peroxide, over 2000 oxidation-sensitive methionines in more than 1600 different proteins were mapped and their extent of oxidation was quantified. Meta-analysis of the sequences surrounding the oxidized methionine residues revealed a high preference for neighboring polar residues. Using synthetic methionine sulfoxide containing peptides designed according to the observed sequence preferences in the oxidized Jurkat proteome, we discovered that the substrate specificity of the cellular methionine sulfoxide reductases is a major determinant for the steady-state of methionine oxidation. This was supported by a structural modeling of the MsrA catalytic center. Finally, we applied our method onto a serum proteome from a mouse sepsis model and identified 35 in vivo methionine oxidation events in 27 different proteins.
ISSN: 1535-9476
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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