Title: Effects of AC-electrolysis on the enzymatic activity of glucose oxidase
Authors: Ammam, Malika ×
Fransaer, Jan #
Issue Date: Mar-2011
Publisher: Wiley-VCH
Series Title: Electroanalysis vol:23 issue:3 pages:755-763
Abstract: The change in the activity of glucose oxidase subjected to an asymmetrical alternating current (AC) electric field is investigated via horseradish peroxidase (HRP)-coupled bioassay. The effect of the amplitude, frequency and enzyme concentration have been shown to affect the enzyme activity towards glucose oxidation. The decrease in the enzyme activity is directly related to the change in pH and temperature of the GOx solution during AC electrolysis. The enzyme activity reduces with increasing amplitude, enzyme concentration and decreasing frequency. Results from UV-vis, FT-IR and UV CD spectroscopy showed that the AC treated GOx samples undergo structural modifications.
ISSN: 1040-0397
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Chemical and Extractive Metallurgy Section (-)
× corresponding author
# (joint) last author

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