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Title: Rapid Method for Lipid Determination of Microalgae
Authors: Ryckebosch, Eline
Muylaert, Koenraad
Foubert, Imogen #
Issue Date: Nov-2010
Conference: EuroFedLipid Congress edition:8 location:Munich, Germany date:21-24 November 2010
Abstract: Microalgae are exponentially growing aquatic organisms that perform photosynthesis very efficiently. As such, they can realize a much higher production per square meter than traditional agricultural plants. Furthermore, they are poor in structural polysaccharides and richer in proteins and lipids. Microalgae have a characteristic lipid composition, with fatty acids often differing from those in higher animal and vegetable organisms.
To screen microalgae for their amount of lipids, an analytical procedure to extract the lipids can be performed. This method is time- and labor-intensive, making it difficult to screen large numbers of algae. The aim of this research was to propose a rapid method for lipid determination of microalgae.
For the development of the rapid method, Nile Red, a lipid-soluble fluorescent dye, was tested. This dye is known to be a fluorescent marker for neutral lipids. Pretreatment of the algal cell suspensions was avoided as much as possible and was therefore limited to dilution of the algal suspension until the OD (720 nm) was between 0.1 and 0.3. The area of the fluorescence peak and height at different emission wavelengths were compared to the actual amount of total and neutral lipids.
Unfortunately, there appears to be no good relationship between the actual amount of the total or neutral lipids and the area of the fluorescence peak or the height of fluorescence at any of the emission wavelengths measured. We can only conclude that the Nile Red method gives some indication about the total and/or neutral lipids present, but cannot be used as a quantitative technique.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Interdisciplinary Research Facility Life Sciences, Campus Kulak Kortrijk
Microbial and Molecular Systems, Campus Kulak Kortrijk
Biology, Campus Kulak Kortrijk
# (joint) last author

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