Title: Phenotypic and genotypic variations within a single bacteriophage species
Authors: Ceyssens, Pieter-Jan
Glonti, Thea
Kropinski, Andrew M
Lavigne, Rob ×
Chanishvili, Nina
Kulakov, Leonid
Lashkhi, Nino
Tediashvili, Marina
Merabishvili, Maya #
Issue Date: Mar-2011
Publisher: BioMed Central
Series Title: Virology Journal vol:8 issue:1 pages:134-1
Abstract: ABSTRACT: BACKGROUND: Although horizontal gene transfer plays a pivotal role in bacteriophage evolution, many lytic phage genomes are clearly shaped by vertical evolution. We investigated the influence of minor genomic deletions and insertions on various phage-related phenotypic and serological properties. FINDINGS: We collected ten different isolates of Pseudomonas aeruginosa bacteriophage phiKMV. All sequenced genomes (42-43 kb, long direct terminal repeats) are nearly identical, which intuitively implied strongly similar infections cycles. However, their latent periods vary between 21 and 28 minutes and they are able to lyse between 5 and 58% of a collection of 107 clinical P. aeruginosa strains. These differences in host spectra were even significant between phages with identical tail structures. Moreover, point mutations in tail and spike proteins were sufficient to evade neutralization by two phage-specific antisera, isolated from rabbits. CONCLUSION: Although all analyzed phages are 83-97% identical at the genome level, they display a surprisingly large variation in various phenotypic properties. The small overlap in host spectrum and their ability to readily escape immune defences against a nearly identical phage are promising elements for the application of these phages in phage therapy.
ISSN: 1743-422X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
× corresponding author
# (joint) last author

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