Applied and Environmental Microbiology vol:77 issue:10 pages:3443-3450
Pantoea agglomerans is a common soil bacterium used in biocontrol of fungi and bacteria, but is also an opportunistic human pathogen. It is extensively described in this context, but knowledge of bacteriophages infecting this species is limited. Bacteriophages LIMEzero and LIMElight of P. agglomerans are lytic phages, isolated from soil samples, belonging to the Podoviridae and are the first described Pantoea-phages of this family. The dsDNA genomes (43,032 bp and 44,546 bp, respectively) encode 57 and 55 ORFs. Based on the presence of an RNA polymerase in their genomes and overall genome architecture, these phages should be classified in the subfamily of the Autographivirinae, within the genus of the 'phiKMV-like viruses'. Phylogenetic analysis of all the sequenced members of the Autographivirinae supports the classification of phages LIMElight and LIMEzero as members of the 'phiKMV-like viruses' and corroborates the subdivision in the different genera. These data expand the knowledge of Pantoea-phages and illustrate the wide host diversity of phages within the 'phiKMV-like viruses'.