Title: Intraperoxisomal redox balance in mammalian cells: oxidative stress and interorganellar crosstalk
Authors: Ivashchenko, Oksana
Van Veldhoven, Paul P
Brees, Chantal
Ho, Ye-Shih
Terlecky, Stanley R
Fransen, Marc # ×
Issue Date: May-2011
Publisher: American Society for Cell Biology
Series Title: Molecular Biology of the Cell vol:22 issue:9 pages:1440-1451
Abstract: Reactive oxygen species (ROS) are at once unsought by-products of metabolism and critical regulators of multiple intracellular signaling cascades. In non-photosynthetic eukaryotic cells, mitochondria are well investigated major sites of ROS generation and related signal initiation. Peroxisomes are also capable of ROS generation, but their contribution to cellular oxidation-reduction (redox) balance and signaling events are far less well understood. In this study, we use a redox-sensitive variant of EGFP (roGFP2-PTS1) to monitor the state of the peroxisomal matrix in mammalian cells. We show that intraperoxisomal redox status is strongly influenced by environmental growth conditions. Furthermore, disturbances in peroxisomal redox balance, although not necessarily correlated with the age of the organelle, may trigger its degradation. We also demonstrate that mitochondrial redox balance is perturbed in catalase-deficient cells and upon generation of excess ROS inside peroxisomes. Peroxisomes are found to resist oxidative stress generated elsewhere in the cell, but are affected when the burden originates within the organelle. These results suggest a potential broader role for the peroxisome in cellular aging and the initiation of age-related degenerative disease.
ISSN: 1059-1524
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Lipid Biochemistry and Protein Interactions
× corresponding author
# (joint) last author

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