Title: Probing the osteoinductive effect of calcium phosphate by using an in vitro biomimetic model
Authors: Chai, Yoke Chin ×
Roberts, Scott
Schrooten, Jan
Luyten, Frank #
Issue Date: Apr-2011
Publisher: Mary Ann Liebert Inc
Series Title: Tissue Engineering Part A vol:17 issue:7-8 pages:1083-1097
Abstract: The use of calcium phosphate (CaP)-based carriers in bone engineering is a promising approach to induce in vivo bone formation. However, the exact mechanism of osteoinduction by CaP is not known. Here, by mimicking the in vivo Ca(2+) and P(i)-enriched environment in an in vitro model, we assessed the effects of these ions on human periosteum-derived cells. We observed a significant Ca(2+) and P(i)-induced cell proliferation, which was found to be through the modulation of cell cycle progression, in a dose- and time-dependent manner. In addition, Ca(2+), P(i), and combined Ca-P upregulated osteogenic gene expression in a dose- and time-dependent manner. Encouragingly, both ions administered individually or in combination persistently and dose dependently upregulated bone morphogenetic protein-2 gene expression. This suggested a potential osteoinductive effect through an autonomous activation of the bone morphogenetic protein signaling pathway by released Ca(2+) and P(i), which may serve as an autocrine/paracrine osteoinduction loop that drives the cellularized CaP constructs toward effective bone formation in vivo. In conclusion, through an in vitro biomimetic model, we have partially probed the roles of the released Ca(2+) and P(i) on the osteoinductivity of CaP-based biomaterials.
ISSN: 1937-3341
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Rheumatology Section (-)
Department of Materials Engineering - miscellaneous
× corresponding author
# (joint) last author

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