Title: DNA Strands from Denatured Duplexes are Translocated through Engineered Protein Nanopores at Alkaline pH
Authors: Maglia, Giovanni
Henricus, Marsiyana
Wyss, Romain
Cheley, Stephen
Bayley, Hagan # ×
Issue Date: Nov-2009
Publisher: American Chemical Society
Series Title: Nano Letters vol:9 issue:11 pages:3831-3836
Abstract: Nanopores are under development for the detection of a variety of analytes and the investigation of chemical reactions at the single molecule level. In particular, the analysis of nucleic acid molecules is under intense investigation, including the development of systems for rapid, low-cost DNA sequencing. Here, we show that DNA can be translocated through an engineered alpha HL protein pore at pH 11.7, a value at which dsDNA is denatured. Therefore, the aHL pore is sufficiently stable to entertain the possibility of direct nanopore sequencing of genomic dsDNA samples, which are more readily obtained and handled than ssDNA.
ISSN: 1530-6984
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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