Identification of epigenetic DNA modifications with a protein nanopore
Wallace, Emma V. B × Stoddart, David Heron, Andrew J Mikhailova, Ellina Maglia, Giovanni Donohoe, Timothy J Bayley, Hagan #
Royal Society of Chemistry
Chemical Communications vol:46 issue:43 pages:8195-8197
Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.