Title: Enhanced translocation of single DNA molecules through alpha-hemolysin nanopores by manipulation of internal charge
Authors: Maglia, Giovanni ×
Restrepo, Marcela Rincon
Mikhailova, Ellina
Bayley, Hagan #
Issue Date: Dec-2008
Publisher: National Academy of Sciences
Series Title: Proceedings of the National Academy of Sciences of the United States of America vol:105 issue:50 pages:19720-19725
Abstract: Both protein and solid-state nanopores are under intense investigation for the analysis of nucleic acids. A crucial advantage of protein nanopores is that site-directed mutagenesis permits precise tuning of their properties. Here, by augmenting the internal positive charge within the alpha-hemolysin pore and varying its distribution, we increase the frequency of translocation of a 92-nt single-stranded DNA through the pore at + 120 mV by approximate to 10-fold over the wild-type protein and dramatically lower the voltage threshold at which translocation occurs, e. g., by 50 mV for 1 event.s(-1).mu M-1. Further, events in which DNA enters the pore, but is not immediately translocated, are almost eliminated. These experiments provide a basis for improved nucleic acid analysis with protein nanopores, which might be translated to solid-state nanopores by using chemical surface modification.
ISSN: 0027-8424
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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