Title: Possible influence of surfactants and proteins on the efficiency of contact agar microbiological surface sampling
Other Titles: efficiency of microbiological surface sampling
Authors: Deckers, Sylvie
Sindic, Marianne
Anceau, Christine ×
Brostaux, Yves
Detry, Jean #
Issue Date: Nov-2010
Publisher: International Association of Milk, Food, and Environmental Sanitarians
Series Title: Journal of Food Protection vol:73 issue:11 pages:2116-2122
Abstract: Agar contact microbiological sampling techniques, based on a transfer of the microorganisms present on a surface to a
culture medium, are widely used to assess and control surface cleanliness and to evaluate microbial contamination levels. The
effectiveness of these techniques depends on many environmental parameters that influence the strength of attachment of the
bacteria to the surface. In the present study, stainless steel and high density polyethylene surfaces were inoculated with known
concentrations of Staphylococcus epidermidis. Following an experimental design, the surfaces were sampled with different types
of replicate organism direct agar contact plates and Petrifilm; results indicated that recovery rates were influenced by the presence
of egg white albumin or Tween 80 in the inoculum solutions or by the introduction of surfactants into the contact agar of the
microbiological sampling techniques. The techniques yielded significantly different results, depending on sampling conditions,
underlining the need for a standardization of laboratory experiments to allow relevant comparisons of such techniques.
ISSN: 0362-028X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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