Title: Label-free imaging of biomolecules in food products using stimulated Raman microscopy
Authors: Roeffaers, Maarten ×
Zhang, Xu
Freudiger, Christian
Saar, Brian
van Ruijven, Marjolein
van Dalen, Gerard
Xiao, Chunhong
Xie, X. Sunney #
Issue Date: Feb-2011
Publisher: Published by SPIE--the International Society for Optical Engineering in cooperation with International Biomedical Optics Society
Series Title: Journal of Biomedical Optics vol:16 issue:2 pages:021118
Article number: 021118
Abstract: The development of methods that allow microscale studies of complex biomaterials based on their molecular composition is of great interest to a wide range of research fields. We show that stimulated Raman scattering (SRS) microscopy is an excellent analytical tool to study distributions of different biomolecules in multiphasic systems. SRS combines the label-free molecular specificity of vibrational spectroscopy with an enhanced sensitivity due to coherent excitation of molecular vibrations. Compared to previous imaging studies using coherent anti-Stokes Raman scattering microscopy, the main advantage of SRS microscopy is the absence of the unwanted nonresonant background, which translates into a superior sensitivity and undistorted vibrational spectra. We compare spectra of complex materials obtained with stimulated Raman scattering and spontaneous Raman scattering in the crowded fingerprint region.We find that, as expected, there is excellent correspondence and that the SRS spectra are free from interference from background fluorescence. In addition, we show high-resolution imaging of the distributions of selected biomolecules, such as lipids and proteins, in food products with SRS microscopy.
ISSN: 1083-3668
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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