Title: Isolation and characterization of expressed sequence tag-linked microsatellite loci for the European eel (Anguilla anguilla)
Authors: Pujolar, J. M ×
Maes, Gregory
Van Houdt, J. K. J
Zane, L #
Issue Date: Jan-2009
Publisher: Wiley-Blackwell
Series Title: Molecular Ecology Resources vol:9 issue:1 pages:233-235
Abstract: A European eel (Anguilla anguilla) expressed sequence tag database consisting of 795 contigs and 4008 singletons was screened for microsatellites sequences. Primers were designed to amplify 96 repeats, of which 86 gave good quality amplification products. Twenty-eight microsatellites were selected for further microsatellite genotyping. Only two loci were found to be monomorphic; out of the 26 polymorphic loci, number of alleles per locus ranged from two to 14, while the observed and expected heterozygosities ranged from 0.05 to 0.93, and from 0.05 to 0.95, respectively. All 28 primer sets tested revealed positive amplification in American eel (Anguilla rostrata).
ISSN: 1755-098X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory for Animal Biodiversity and Systematics (-)
Ecology, Evolution and Biodiversity Conservation Section
× corresponding author
# (joint) last author

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