Title: Impact of pre-concentration to covalently biofunctionalize suspended nanoparticles
Authors: Jans, Hilde ×
Jans, Karolien
Stakenborg, Tim
Van de Broek, Bieke
Lagae, Liesbet
Maes, Guido
Borghs, Gustaaf #
Issue Date: Aug-2010
Publisher: IOP Pub.
Series Title: Nanotechnology vol:21 issue:34
Article number: 345102
Abstract: The effective biofunctionalization of nanoparticles is crucial for biomedical applications. In this study we investigated the covalent biofunctionalization of magnetic nanoparticles based on carbodiimide activation. An important aspect in the covalent biofunctionalization of nanoparticles has been neglected, namely pre-concentration. Exploiting the electrostatic attraction forces between a protein and the nanoparticle surface will favor the covalent immobilization. We showed that low ionic strength buffers with a pH slightly lower than the pI of the selected biomolecules is needed to increase the yield of covalent immobilization. Additionally, it is demonstrated that the covalently immobilized proteins are bioactive, relying on a sandwich assay using gold nanoparticles as reporter labels.
ISSN: 0957-4484
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Quantum Chemistry and Physical Chemistry Section
Solid State Physics and Magnetism Section
Semiconductor Physics Section
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science