Title: TRIzol treatment of secretory phase endometrium allows combined proteomic and mRNA microarray analysis of the same sample in women with and without endometriosis
Authors: Fassbender, Amelie ×
Simsa, Peter
Kyama, Cleophas M
Waelkens, Etienne
Mihalyi, Attila
Meuleman, Christel
Gevaert, Olivier
Van de Plas, Raf
De Moor, Bart
D'Hooghe, Thomas #
Issue Date: Oct-2010
Publisher: BioMed Central
Series Title: Reproductive Biology and Endocrinology vol:8 pages:123-123
Abstract: According to mRNA microarray, proteomics and other studies, biological abnormalities of eutopic endometrium (EM) are involved in the pathogenesis of endometriosis, but the relationship between mRNA and protein expression in EM is not clear. We tested for the first time the hypothesis that EM TRIzol extraction allows proteomic Surface Enhanced Laser Desorption/Ionisation Time-of-Flight Mass Spectrometry (SELDI-TOF MS) analysis and that these proteomic data can be related to mRNA (microarray) data obtained from the same EM sample from women with and without endometriosis.
ISSN: 1477-7827
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Reproductive Medicine Section (-)
Section Woman - Miscellaneous (-)
ESAT - STADIUS, Stadius Centre for Dynamical Systems, Signal Processing and Data Analytics
Laboratory of Protein Phosphorylation and Proteomics
Laboratory of Phosphoproteomics (-)
× corresponding author
# (joint) last author

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