Title: Activation of TRPV4 channels reduces migration of immortalized neuroendocrine cells
Authors: Zaninetti, Roberta ×
Fornarelli, Alessandra
Ciarletta, Monica
Lim, Dmitry
Caldarelli, Antonio
Pirali, Tracey
Cariboni, Anna
Owsianik, Grzegorz
Nilius, Bernd
Canonico, Pier Luigi
Distasi, Carla
Genazzani, Armando A #
Issue Date: Feb-2011
Publisher: Raven Press
Series Title: Journal of Neurochemistry vol:116 issue:4 pages:606-15
Article number: 10.1111/j.1471-4159.2010.07144.x
Abstract: J. Neurochem. (2011) 116, 606-615. ABSTRACT: Calcium is a universal signal, and its capacity to encode intracellular messages via spatial, temporal and amplitude characteristics allows it to participate in most cellular events. In a specific context, calcium plays a pivotal role in migration, although its role has not been elucidated fully. By using immortalized gonadotropin-releasing hormone-secreting neurons (GN11), we have now investigated the role of TRPV4, a member of the vanilloid family of Ca(2+) channels, in neuronal migration. Our results show that TRPV4 channels are present and functional in GN11 cells and their localization is polarized and enriched in lamellipodial structures. TRPV4 activation leads to a retraction of the lamellipodia and to a decrease in migratory behaviour; moreover cells migrate slower and in a more random manner. We therefore provide evidence for a new regulation of gonadotropin-releasing hormone neurons and a new role for calcium at the leading edge of migratory cells.
ISSN: 0022-3042
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Cellular and Molecular Medicine - miscellaneous
Laboratory of Ion Channel Research
× corresponding author
# (joint) last author

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