Title: Purification of human fibroblast interferon by zinc chelate affinity chromatography
Authors: Edy, V G ×
Billiau, Alfons
de Somer, P #
Issue Date: Sep-1977
Series Title: Journal of Biological Chemistry vol:252 issue:17 pages:5934-5
Abstract: Zinc chelated by iminodiacetic acid linked to an insoluble matrix binds human fibroblast interferon quite selectively at neutral pH in 0.15 M NaCL. On reduction of the pH and increase of the ionic strength, the interferon is eluted. Using a pH gradient at constant high ionic strength, good purification of the interferon can be obtained, up to a final specific activity of 108.5 units/mg of protein. Approximately 60% of the applied activity can be recovered.
ISSN: 0021-9258
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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