Title: Human hepatoma cells produce an 85 kDa gelatinase regulated by phorbol 12-myristate 13-acetate
Authors: Masure, S ×
Billiau, Alfons
Van Damme, Jozef
Opdenakker, Ghislain #
Issue Date: Sep-1990
Series Title: Biochimica et Biophysica Acta vol:1054 issue:3 pages:317-25
Abstract: Several human cell lines were studied for the production of gelatinases. Diploid fibroblasts, the melanoma cell line Bowes, the MG-63 osteosarcoma cell line and the human hepatoma cell line Malavu all constitutively produced a 67 kDa gelatinase. Gelatinolytic enzymes were quantified by a sensitive zymographic substrate conversion assay. Upon induction with phorbol 12-myristate 13-acetate (PMA), the human hepatoma cell line secreted considerable amounts of an 85 kDa gelatinase activity. The induction process was time- and dose-dependent. It represented a true increase in production per individual cell and was associated by a marked change of the cell morphology. The effect of various proteinase inhibitors and the maximal activity of the enzyme near neutral pH demonstrate that it is a neutral metalloproteinase. Characterization studies showed the 85 kDa gelatinase to be transformed to lower molecular weight, active forms by treatment with p-aminophenylmercuric acetate (APMA) or trypsin.
ISSN: 0006-3002
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Immunology (Rega Institute)
Laboratory of Immunobiology (Rega Institute)
Academic Center for General Practice
× corresponding author
# (joint) last author

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