Title: Influence of castration and androgen treatment on the synthesis of prostatic binding protein and the concentration of its mRNA in the rat ventral prostate
Authors: Bossyns, D ×
Delaey, B
Rombauts, Wilfried
Heyns, Walter #
Issue Date: Mar-1986
Series Title: Journal of steroid biochemistry vol:24 issue:3 pages:661-8
Abstract: The regulation by androgens of the synthesis of prostatic binding protein (PBP), an abundant secretory protein of the rat ventral prostate, was studied by measuring the rate of incorporation of [3H]leucine into total protein and into PBP by prostatic tissue in vitro. PBP-specific synthesis dropped to 30% of the intact level after 3 days and to 5.5% after 7 days. Administration of androgens to 7 day-castrated rats reversed these changes: 36% of the control value was reached after 3 days of treatment and 70% after 5 days. The mRNAs of PBP were measured under similar conditions by means of dot hybridization, using C1-, C2- and C3-specific cDNA-clones. The 3 mRNAs followed a parallel course. They decreased to about 18% on the third day after castration and to about 2% after 7 days. Androgen treatment of 7-day castrated rats produced a small increase of these levels after 1 day. After 3 days 15% of the intact level was reached. The general similarity of the time course of PBP synthesis and PBP mRNA levels in castrated and androgen treated rats supports a transcriptional regulation mechanism, although there also is some evidence for an influence on protein synthesis in general.
ISSN: 0022-4731
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry Section (Medicine) (-)
Clinical and Experimental Endocrinology
× corresponding author
# (joint) last author

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