Title: Glucose tolerance and pressure-volume relationship in frequently used mouse strains
Authors: Oosterlinck, Wouter
Vanderper, Annelies
Flameng, Willem
Herijgers, Paul #
Issue Date: 2010
Conference: ISHR World Congress location:Kyoto Japan date:12/5/2010-17/5/2010
Article number: 70346
Abstract: We investigated glucose tolerance and left ventricular
performance in 4 mice background strains (Swiss, C57BL6/J,
DBA2 and BalbC) at 24 weeks. Glucose tolerance was tested by
intraperitoneal injection of 2mg/g body weight of glucose. Load
dependent and-independent contractility parameters were
assessed by left ventricular pressure-conductance analysis
(Millar, PVAN3.6) under urethane (1.2 g/kg) and alfa-chloralose
(50 mg/kg) anaesthesia. Dose-responsiveness to Isoprenaline was
determined subsequently. Peak glucose levels and area under the
curve (AUC), were higher in C57BL6/J (418±65mg/dl and
813±100mg.h/dl) versus Swiss (237±66mg/dl, p<10-6 and
470±126mg.h/dl, p=0,001), DBA2 (113±20mg/dl, p=5.10-6and
304±49mg.h/dl, p=0,001) and BalbC mice (174±55mg/dl, p<10-6
and 416±70mg.h/dl, p=6.10-4). Cardiac output and dP/dtmax were
higher in Swiss (14038 ±4530μl/min and 11746±2105 mmHg/s)
versus C57BL6/J (10405±2683 μl/min, p=0,003 and 7162±1563
mmHg/s, p=10-6), DBA2 (10438±3251μl/min, p=0,008 and
9420±2268 mmHg/s, p=0,01) and BalbC mice (8466±
3013μl/min, p=10-4and 7760±1581 mmHg/s, p=5.10-5). Preload
recruitable stroke work (PRSW) was not different. Under
Isoprenaline, BalbC mice showed the largest heart rate increase
(30% in BalbC mice vs 16% in Swiss, 20% in C57BL6/J, and
23% in DBA2 mice), while PRSW did not change significantly.
In conclusion, glucose tolerance and cardiac performance
parameters were significantly different between 4 mice
background strains. However, load independent contractility
parameters were similar.
Publication status: published
KU Leuven publication type: IMa
Appears in Collections:Experimental Cardiac Surgery
# (joint) last author

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